PROTOCOLS​

Biochemistry

MAC purification of recombinant proteins

Western to check for His Tagged proteins

Protein expression in E. coli

CD thermal denaturation

Bradford Microassay

MALDI Matrix Guide

Molecular Biology

Preparing and Transforming Electro-Competent Cells

Transformation of DNA Targets into Bacillus Subtilis

DNA Purification Through Gel Extraction

Preparing Chemically Competent Cells

Plasmid Mini-Preps via P1, P2, and P3

Splice by Overlap Extension

Site-Directed Mutagenesis

Molecular Cloning

Microbiology

Extraction of Metabolites from Bacterial Cell Culture (liquid-liquid)

MIC Determination by Microtitre Broth Dilution Method

Solid Phase Extraction of Metabolites

Preparing Antibiotic Stock Solutions

Prep of Bacterial Long Term Stocks

M. xanthus culture media

Minimal Media

LB Plates

​Organic Chemistry

SNAC Synthesis from Cysteamine Hydrochloride

Solvent Purification System

Thin Layer Chromatography

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SECONDARY METABOLITES RESOURCES 

Web Tools

antiSMASH

BiG-SCAPE

autoMLST

NaPDoS

RiPPER

ARTS

GNPS

(Meta)Genome Tools

BAGEL4

PATRIC

RAST

Repositories

MIBiG

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ADMINISTRATIVE DOCUMENTS​

Mass Spec Submission Forms (Personalizable)

Hazardous Waste Collection Online Form

Autoclave Request Form

Science Store Signing Delegation Form

Used Barcode Return Form

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EQUIPMENT BOOKING

LC-MS/MS & MALDI Scheduling

NMR Scheduling