< Publications



Whole genome sequencing and metabolomic study of cave Streptomyces isolates ICC1 and ICC4. Jessica T. Gosse, Soumya Ghosh, Amanda Sproule, David P. Overy, Naowarat Cheeptham, Christopher N. Boddy Front. Microbiol. 2019. doi: 10.3389/fmicb.2019.01020

Trapping biosynthetic acyl-enzyme intermediates with encoded 2,3-diaminopropionic acidNicolas Huguenin-Dezot, Diego A. Alonzo, Graham W. Heberlig, Mohan Mahesh, Duy P. Nguyen, Mark H. Dornan, Christopher N. Boddy, T. Martin Schmeing & Jason W. Chin Nature2019, 565, 112-117doi:10.1038/s41586-018-0781-z


Draft genome of the type strain Streptomyces armeniacus ATCC 15676. Labana, P.; Gosse, J. T.; Boddy, C. N. Microbiol. Resour. Announc. 2018, 7, e01107-18.  

Chemoenzymatic Macrocycle Synthesis Using Resorcylic Acid Lactone Thioesterase Domains. Heberlig, G.W.; Brown, J.T.C.; Simard, R.; Wirz, M.; Zhang, W.; Wang, M.; Susser, L.I.; Horsman, M.E.; Boddy, C.N., Org. Biomol. Chem. 2018, 16, 5771-5779 doi: 10.1039/C8OB01512K

Inihibition of Bacterial Gene Transcription with an RpoN-based Stapled Peptide. Sterling R. Payne1, Daniel I. Pau1, Ye Joon Kim, Amanda L. Whiting, Blaze M. Pharoah, Christina A. Moi, Christopher N. Boddy, Federico Bernal Cell Chemical Biology 2018, 25, 1-8. doi:10.1016/j.chembiol.2018.05.007
1Equal Contribution


Sampling Terrestrial Environments for Bacterial PolyketidesPatrick Hill, Graham W. Heberlig, Christopher N. Boddy Molecules 2017, 22, 707-756. doi:10.3390/molecules22050707. 

Inducible T7 RNA polymerase-mediated multigene expression system, pMGX.  Hassan, M. I; McSorley, F. R.; Hotta, K.; Boddy, C. N. J. Vis. Exp. 2017 (124), e55187, DOI:10.3791/55187 video

Natural products: Mapping an amazing thicket. Mark E. Horsman, Christopher N. Boddy Nature Chem. Biol. 201713, 6-7. DOI: 10.1038/nchembio.2265.


Portable, On-Demand Biomolecular Manufacturing. Keith Pardee, Shimyn Slomovic, Peter Q. Nguyen, Jeong Wook Lee, Nina Donghia, Devin Burrill, Tom Ferrante, Fern R. McSorley, Yoshikazu Furuta, Andyna Vernet, Michael Lewandowski, Christopher N. Boddy, Neel S. Joshi, James J. Collins, Cell 2016, 167, 248-259.  DOI: 10.1016/j.cell.2016.09.013

Total Biosynthesis of Legionaminic Acid, a Bacterial Sialic Acid Analog. Hassan, M. I.; Lundgren, B. L.; Chaumun, M.; Whitfield, D. M.; Clark, B.; Schoenhofen, I. C.; Boddy, C. N. Angew. Chem. Int. Ed. 201655, 12018-12021. DOI: 10.1002/anie.201606006

First-in-class small molecule potentiators of cancer virotherapy.  Dornan, M. H.; Krishnan, R.; Macklin, A. M.; Selman, M.; El Sayes, N.; Son, H. H.; Davis, C.; Chen, A.; Keillor, K.; Le, P. J.; Moi, C.; Ou, P.; Pardin, C.; Canez, C. R.; Le Boeuf, F.; Bell, J. C.; Smith, J. C.; Diallo J.-S.; Boddy C. N. Sci. Rep. 2016, 6, 26786. DOI: 10.1038/srep26786

N-Acetylneuraminic acid production in Escherichia coli lacking N-acetylglucosamine catabolic machinery. Horsman, M. E.; Lundgren, B. L.; Boddy, C. N. Chem. Eng. Commun. 2016, 203. 1326-1335. DOI: 10.1080/00986445.2016.1188293

The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways. Tremblay, N.; Hill, P.; Conway, K.; R; Boddy, C. N. Meth. Mol. Biol. 2016, 233-252. ISBN: 978-1-4939-3375-4


Diastereoselective Transannular Oxa-conjugate Addition Generates the 2,6-cis-Disubstituted Tetrahydropyran of Neopeltolide.  Hari, T. P.; Wilke, B. I.; Davey, J.; Boddy, C. N. J. Org. Chem. 201581(2), 415-423. DOI: 10.1021/acs.joc.5b02014

Towards a characterization of the structural determinants of specificity in the macrocyclizing thioesterase for deoxyerythronolide B biosynthesis. Argyropoulos, P.; Bergeret, F.; Pardin, P.; Reimer J. M.; Pinto, A.; Boddy, C. N.; Schmeing, M. BBA - General Subjects 2015, 1860, 486-497. DOI: 10.1016/j.bbagen.2015.11.007

Draft Genome Sequence of Streptomyces sp. Strain PBH53, isolated from urban environment. Gosse, J. T.; Hill, P.; Dowd, S. E.; Boddy, C. N. Genome Announc. 2015, 3, e00859-15, DOI:  10.1128/genomeA.00859-15.

Thermoplasmonic ssDNA Dynamic Release from Gold Nanoparticles Examined with Advanced Fluorescence Microscopy. Simoncelli, S.; de Alwis Weerasekera, H.; Fasciani, C.; Boddy, C. N.; Aramendía, P.; Alarcon, E.; Scaiano, J. J. Phys. Chem. Lett. 2015, 6 1499-1503, DOI:  10.1021/acs.jpclett.5b00272.

Polyketide Synthase and Non-Ribosomal Peptide Synthetase Thioesterase Selectivity: Logic Gate or a Victim of Fate? Horsman, M. E.; Hari, T. P. A; Boddy, C. N. Nat. Prod. Rep. 2015. DOI: 10.1039/c4np00148f.

Modulation of antifreeze activity and the effect upon post-thaw HepG2 cell viability after cryopreservation. Capicciotti, C. J.; Poisson, J. S.; Boddy, C. N.; Ben, R. N. Cryobiology 2015, DOI: 10.1016/j.cryobiol.2015.01.002.


Resorcylic acid lactone biosynthesis relies on a stereo-tolerant macrocyclizing thioesteraseHeberlig, Graham; Wirz, Monica; Wang, Meng; Boddy, Christopher N. Org. Lett. 2014, 16, 5858-5861.

Salvadenosine, a 5'-Deoxy-5'-methylthio Nucleoside from the Bahamian Tunicate, Didemnum sp. Jamison, M. T.; Boddy, C. N.; Molinski, T. F. J. Org. Chem. 2014, 79, 9992–9997.

An evolutionary model encompassing substrate specificity and reactivity of type I polyketide synthase thioesterases. Hari, Taylor P.A.; Labana, Puneet; Boileau, Meaghan; Boddy, Christopher N., ChemBioChem 2014, 15, 2656-61. DOI: 10.1002/cbic.201402475.

Genetic Analysis of the Assimilation of C-5-Dicarboxylic Acids in Pseudomonas aeruginosa PAO1. Lundgren, B.R.; Villegas-Peñaranda, L. R.; Harris, J. R.; Mottern, A. M.; Dunn, D. M.; Boddy, C. N.; Nomura, C. T. Journal of Bacteriology 2014, Volume 196, Issue 14, Pages 2543-2551. DOI: 10.1128/JB.01615-14

Bioinformatics tools for genome mining of polyketide and non-ribosomal peptides. Boddy C.N. Journal of Industrial Microbiology & Biotechnology 2014, Volume 41, Issue 2  Special Issue SI,   Pages 443-450. DOI: 10.1007/s10295-013-1368-1.

Hexanes/acetonitrile: A binary solvent system for the efficient monosilylation of symmetric primary and secondary diols. Wilke, Burkhardt I.; Dornan, Mark H.; Yeung, Jon; Boddy, Christopher N.; Pinto, Atahualpa. Tetrahedron Lett. 2014, 55, 2600–2602. DOI: 10.1016/j.tetlet.2014.02.067


Elucidation of Gephyronic Acid Biosynthetic Pathway Revealed Unexpected SAM-Dependent Methylations. Young, Jeanette; Stevens, D. Cole; Carmichael, Rory; Tan, John; Rachid, Shwan; Boddy, Christopher N.; Müller, Rolf; Taylor Richard E. J. Nat. Prod. 201376 (12), pp 2269–2276 doi: 10.1021/np400629v

Habitat specific type I Polyketide synthases in soils and street sediments.  Hill, Patrick; Piel, Jörn; Aris-Brosou, Stéphane; Krištůfek, Václav; Boddy, Christopher N.; Dijkhuizen, Lubbert. J. Ind. Microbiol. Biotechnol. 2013doi: 10.1007/s10295-013-1343-x

The role of transcription in heterologous expression of polyketides in
bacterial hosts.
Stevens, David C.; Hari, Taylor P. A.; Boddy, Christopher N. Nat. Prod. Rep. 201330, 1391-1411. doi: 10.1039/C3NP70060G

Biosynthesis of ebelactone A: isotopic tracer, advanced precursor and genetic studies reveal a thioesterase-independent cyclization to give a polyketide β-lactone. Wyatt, Morgan A.; Ahilan, Yasodha; Argyropoulos, Panos; Boddy, Christopher N.; Magarvey, Nathan A.; - Harrison, Paul H. M. J. Antibiot. 2013, 66, 421.  doi: 10.1038/ja.2013.48

Alternative Sigma Factor Over-Expression Enables Heterologous Expression of a Type II Polyketide Biosynthetic Pathway in Escherichia coli. Stevens, David C.; Conway, Kyle; Pearce, Nelson; Villegas-Peñaranda, Luis R.; Garza, Anthony G.; Boddy, Christopher N. PLoS ONE 20138, e64858. doi: 10.1371/journal.pone.0064858

The Gene PA2449 is Essential for Glycine Metabolism and Pyocyanin Biosynthesis in Pseudomonas aeruginosa PAO1.  Lundgren, Benjamin; Thornton, William; Dornan, Mark; Villegas-Peñaranda, Luis; Boddy, Christopher N.; Nomura, Christopher J. Bacteriol. 2013195, 2087. doi: 10.1128/JB.02205-12.

ClusterMine360: a database of Microbial PKS/NRPS Biosynthesis. Conway, Kyle; Boddy, Christopher N. Nucleic Acid Res. 2013, D402. doi: 10.1093/nar/gks993.


Non-canonical regioisomerizations and a 'Diels-Alderase' are likely essential in the biosynthesis of Spiculoic acid A. Pinto, Atahualpa; Boddy, Christopher N. Bioorg Med Chem Lett. 2012, 22, 5253.

6-deoxyerythronolide B synthase thioesterase-catalyzed macrocyclization activity is highly stereoselective. Pinto, Atahualpa; Wang, Meng; Horsman, Mark; Boddy, Chirstopher N. Org. Lett. 2012, 14, 2278.


Coenzyme Q10 Production in the Filamentous Basidiomycete Sporidiobolus johnsonii. Dixson, D.; Boddy, Christopher N.; Doyle, Robert P. Chem. BioDiv. 2011, 8, 1033.

Land use intensity controls Actinobacterial community structure. Hill, Patrick; Krištůfek, Václav; Dijkhuizen, Lubbert; Boddy, Christopher; Kroetsch, David; van Elsas, Jan Dirk Microb. Ecol. 2011, 61, 286.


Process Improvements for the Manufacture of Tenofovir Disoproxyl Fumarate (TDF) at Commercial Scale. Ripin, David H. Brown; Teager, David; Fortunak, Joseph; Basha, K.; Bivins, Nylea; Boddy, Christopher N.; Byrn, Stephen; Catlin, Kelly; Houghton, Stephen R.; Jagadeesh, S. Tirumala; Kumar, K. Anesh; Melton, Jack; Muneer, K.; Rao, L. Nagaprasada; Rao, R. Venkateswara; Reddy, N. Gopal; Reddy, R. Mallikarjuna; Shekar, K. Chandra; Silverton, Tricia; Smith, Daniel T.; Stringham, Rodger; Talley, Frajovon; Williams, Adrian. Org. Process Res. Dev. 2010, 14, 1194.

Rapid, mild method for phosphonate diester hydrolysis: Development of a one-pot synthesis of tenofovir disoproxil fumarate from tenofovir diethyl ester. Houghton, Stephen R.; Melton, Jack; Fortunak, Joseph; Ripin, David H. Brown; Boddy, Christopher N. Tetrahedron 2010, 66, 8137.

Heterologous expression of the oxytetracycline biosynthetic pathway in Myxococcus xanthus. Stevens, David C.; Henry, Michael R.; Murphy, Kimberly; Boddy, Christopher N. Applied Environ. Microbiol. 2010, 76, 2811.



Examining the role of hydrogen bonding interactions in the substrate specificity for the loading step of polyketide synthase thioesterase domains. Wang, Meng; Boddy, Christopher N. Biochemistry 2008, 45, 11793.

Orthogonal ligation: a three piece assembly of a PNA-peptide-PNA conjugate. Burlina, Fabienne; Dixson, David D.; Doyle, Robert P.; Chassaing, Gérard; Boddy, Christopher N.; Dawson, Philip; Offer, John Chem. Commun. 2008, 2785.


A New Mechanism for Benzopyrone Formation in Aromatic Polyketide Biosynthesis. Zhang, Wenju; Wilke, Burkhardt I.; Zhan, Jixun; Watanabe, Kenji; Boddy, Christopher N.; Tang, Yi J. Am. Chem. Soc. 2007, 129, 9304.

Sialic acid and N-acyl sialic acid analog production by fermentation of metabolically and genetically engineered Escherichia coli. Lundgren, Benjamin L.; Boddy, Christopher N. Org. Biomol. Chem. 2007, 5, 1903.

The thioesterase domain from the pimaricin and erythromycin biosynthetic pathways can catalyze hydrolysis of simple thioester substrates. Sharma, Krishna K.; Boddy, Christopher N. Bioorg. Med. Chem. Lett. 2007, 17, 3034.


Total biosynthesis of antitumor nonribosomal peptides in Escherichia coli. Watanabe, Kenji; Hotta, Kinya; Praseuth, Alex P.; Kotetsu, Kento; Migita, Akira; Boddy, Christopher N.; Wang, Clay C. C.; Oguri, Hiroki; Oikawa, Hideaki Nature Chemical Biology 2006, 2, 423.